The Sequencer displays a collection of keyframes of your colored image channels. You can create a movie or assemble a collection of channel perspectives using the timeline. With dynamic functions, you can have blinking pictures, 3D landscapes, and fluid transitions between scenes that give you the ability to fly over the tissue. This makes it easy to view many color channels at once.
Create a Scene
To add a scene to the timeline, or create one for the first time, click the + next to the Working Configuration (or active scene).
Each newly added scene will appear to the left of your working configuration. As you create different channel perspectives, make sure to click the + to save it and add it to the timeline. Double-clicking on a previous scene will revert any progress made within the working configuration.
Working With the Timeline
Click on a scene to select it (Note that if you double click the thumbnail, it becomes the active working configuration).
Add a title to the scene by double-clicking above the scene thumbnail (next to the scene number).
Set the duration of each scene by inputting a numerical value in the seconds box below the thumbnail.
Adjusting the Time Multiplier can also shorten or lengthen the overall duration (default value is always 1). This a quick way to cap the time limit so that you can reliably produce a movie that will, for example, not exceed a 30 second limit for a presentation.
Reorder scenes by dragging and dropping into the desired slot.
If you made any adjustments to the display or navigation settings for a scene, you can update the scene by right-clicking it and selecting Update.
To remove that scene, select remove. If you would like to reset the entire timeline and start anew, select Remove All.
Preview your movie by utilizing the playback options.
You can play the movie by clicking Start Sequencer Playback.
Select Reset Playback to set the current configuration to scene 1. You can initiate the playback at any scene by having it selected as the working configuration.
Move Back Playback and Forward Sequencer Playback buttons will shift your current working configuration back or forward one scene respectively.
Pause any movie from playing by selecting Stop during play-through.
Toggling the Loop will play the movie consecutively until stopped manually.
You can create a movie using a preset available in the favorites dropdown. Click the heart on the top right to view the list.
All z levels
All standard perspectives (from the channels list)
All signaling perspectives (from the channels list)
All regions of interest
Selecting a preset will quickly fill up the timeline with scenes.
Here is an example of a sequencer movie showing tissue loss over 12 rounds:
Click on the top right icon to view the export preferences. You can export the scenes as a video and as high resolution still images.
You can choose to display the legend and allow the maximum image quality when compressing the images. Having Show settings on export again checked will prompt a dialogue box to confirm the settings again when you export.
Once the preferences are set to your liking, export the movie to any directory folder as image files (.Tiff, .JPG, .PNG), or as video files (.mp4, .avi).